Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 57168..57825 | Replicon | plasmid pCREC-591_3 |
| Accession | NZ_CP024824 | ||
| Organism | Escherichia coli strain CREC-591 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | CU078_RS28505 | Protein ID | WP_000270043.1 |
| Coordinates | 57168..57518 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | CU078_RS28510 | Protein ID | WP_000124640.1 |
| Coordinates | 57523..57825 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CU078_RS28455 | 52237..52416 | - | 180 | Protein_58 | winged helix-turn-helix domain-containing protein | - |
| CU078_RS28460 | 52723..53427 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| CU078_RS28465 | 53642..54139 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| CU078_RS28470 | 54142..54630 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| CU078_RS28475 | 54727..55062 | + | 336 | WP_000683476.1 | hypothetical protein | - |
| CU078_RS28480 | 55077..55547 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| CU078_RS28485 | 55540..55911 | - | 372 | WP_000516916.1 | hypothetical protein | - |
| CU078_RS28490 | 55922..56116 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| CU078_RS28500 | 56457..57005 | - | 549 | WP_001061574.1 | transcriptional regulator | - |
| CU078_RS28505 | 57168..57518 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| CU078_RS28510 | 57523..57825 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| CU078_RS28515 | 57852..58145 | - | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| CU078_RS28520 | 58233..58505 | - | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| CU078_RS28525 | 58563..59090 | - | 528 | WP_001236377.1 | thermonuclease family protein | - |
| CU078_RS28535 | 59321..60178 | - | 858 | WP_001167032.1 | hypothetical protein | - |
| CU078_RS28540 | 60165..60395 | - | 231 | WP_000972663.1 | hypothetical protein | - |
| CU078_RS28545 | 60395..60913 | - | 519 | WP_000210756.1 | nitrite reductase | - |
| CU078_RS28550 | 60910..61356 | - | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| CU078_RS28555 | 61356..61715 | - | 360 | WP_000422768.1 | hypothetical protein | - |
| CU078_RS28560 | 61772..62200 | - | 429 | WP_000591074.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR | - | 1..62815 | 62815 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T88673 WP_000270043.1 NZ_CP024824:57168-57518 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T88673 NZ_CP024824:57168-57518 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT88673 WP_000124640.1 NZ_CP024824:57523-57825 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT88673 NZ_CP024824:57523-57825 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|