Detailed information of TA system TA088669

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41158..41428
• Replicon: plasmid pCREC-591_2
• Accession: NZ_CP024823
• Organism: Escherichia coli strain CREC-591

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CU078_RS27955
• Protein ID: WP_001312861.1
• Coordinates: 41270..41428 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41158..41221 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU078_RS27930	36869..37396	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CU078_RS27935	37454..37687	+	234	WP_000006003.1	DUF905 family protein	-
CU078_RS27940	37748..39771	+	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
CU078_RS27945	39840..40274	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CU078_RS27950	40271..40990	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	41002..41226	+	225	NuclAT_0	-	-
-	41002..41226	+	225	NuclAT_0	-	-
-	41002..41226	+	225	NuclAT_0	-	-
-	41002..41226	+	225	NuclAT_0	-	-
CU078_RS29230	41011..41190	-	180	WP_001309233.1	hypothetical protein	-
-	41158..41221	-	64	-	-	Antitoxin
CU078_RS27955	41270..41428	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CU078_RS29500	41666..42043	-	378	Protein_52	hypothetical protein	-
CU078_RS27975	42343..42639	+	297	WP_001272251.1	hypothetical protein	-
CU078_RS27980	42750..43571	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CU078_RS27985	43868..44515	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CU078_RS27990	44792..45175	+	384	WP_000124981.1	relaxosome protein TraM	-
CU078_RS27995	45366..46052	+	687	WP_015059009.1	PAS domain-containing protein	-
CU078_RS28000	46146..46373	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..64564	64564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88669 WP_001312861.1 NZ_CP024823:41270-41428 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88669 NZ_CP024823:41270-41428 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT88669 NZ_CP024823:c41221-41158 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin