Detailed information of TA system TA088661

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46516..46780
• Replicon: plasmid pCREC-591_1
• Accession: NZ_CP024822
• Organism: Escherichia coli strain CREC-591

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: CU078_RS27170
• Protein ID: WP_001331364.1
• Coordinates: 46628..46780 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	46516..46578 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU078_RS27155	41755..44046	-	2292	WP_001289276.1	hypothetical protein	-
CU078_RS27160	44039..45109	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
CU078_RS27165	45128..46336	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	46516..46578	-	63	NuclAT_0	-	Antitoxin
-	46516..46578	-	63	NuclAT_0	-	Antitoxin
-	46516..46578	-	63	NuclAT_0	-	Antitoxin
-	46516..46578	-	63	NuclAT_0	-	Antitoxin
CU078_RS27170	46628..46780	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
CU078_RS27175	46852..47103	-	252	WP_001291964.1	hypothetical protein	-
CU078_RS29480	47762..47938	-	177	WP_072745605.1	hypothetical protein	-
CU078_RS27180	48330..48539	+	210	WP_039025903.1	HEAT repeat domain-containing protein	-
CU078_RS27185	48611..49273	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
CU078_RS27190	49344..51512	-	2169	WP_000698354.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-123 / mph(A) / fosA3	-	1..118156	118156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T88661 WP_001331364.1 NZ_CP024822:46628-46780 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T88661 NZ_CP024822:46628-46780 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT88661 NZ_CP024822:c46578-46516 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin