Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4951319..4951821 Replicon chromosome
Accession NZ_CP024821
Organism Escherichia coli strain CREC-591

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CU078_RS25750 Protein ID WP_000767829.1
Coordinates 4951567..4951821 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CU078_RS25745 Protein ID WP_001259255.1
Coordinates 4951319..4951570 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU078_RS25715 4946498..4947565 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CU078_RS25720 4947565..4948635 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
CU078_RS25725 4948632..4949936 - 1305 WP_001515511.1 histidinol dehydrogenase -
CU078_RS25730 4949942..4950841 - 900 WP_061093169.1 ATP phosphoribosyltransferase -
CU078_RS25740 4950987..4951037 - 51 WP_001364200.1 his operon leader peptide -
CU078_RS25745 4951319..4951570 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CU078_RS25750 4951567..4951821 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CU078_RS25755 4951904..4952728 + 825 WP_000754763.1 SDR family oxidoreductase -
CU078_RS25760 4952774..4953703 + 930 WP_000803351.1 LysR family transcriptional regulator -
CU078_RS25765 4953918..4953980 + 63 WP_010723108.1 membrane protein YoeI -
CU078_RS25770 4953970..4955328 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CU078_RS25775 4955507..4956565 + 1059 WP_023568741.1 transport protein YeeE -
CU078_RS25780 4956579..4956806 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88658 WP_000767829.1 NZ_CP024821:4951567-4951821 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88658 NZ_CP024821:4951567-4951821 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88658 WP_001259255.1 NZ_CP024821:4951319-4951570 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88658 NZ_CP024821:4951319-4951570 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References