Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1892043..1892584 Replicon chromosome
Accession NZ_CP024821
Organism Escherichia coli strain CREC-591

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag CU078_RS10185 Protein ID WP_000615982.1
Coordinates 1892306..1892584 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CU078_RS10180 Protein ID WP_000729704.1
Coordinates 1892043..1892303 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU078_RS10155 1887079..1888218 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
CU078_RS10160 1888202..1890295 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
CU078_RS10165 1890400..1890678 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CU078_RS10170 1890671..1891027 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CU078_RS10175 1891084..1891857 - 774 WP_000543899.1 C40 family peptidase -
CU078_RS10180 1892043..1892303 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CU078_RS10185 1892306..1892584 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CU078_RS10190 1892740..1893480 + 741 WP_001225679.1 murein L,D-transpeptidase -
CU078_RS10195 1893451..1894218 - 768 WP_000333380.1 class II glutamine amidotransferase -
CU078_RS10200 1894424..1895002 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T88647 WP_000615982.1 NZ_CP024821:1892306-1892584 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T88647 NZ_CP024821:1892306-1892584 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT88647 WP_000729704.1 NZ_CP024821:1892043-1892303 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT88647 NZ_CP024821:1892043-1892303 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References