Detailed information of TA system TA088625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3363158..3363720
• Replicon: chromosome
• Accession: NZ_CP024819
• Organism: Citrobacter freundii strain CRCB-101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CU079_RS16305
• Protein ID: WP_100194942.1
• Coordinates: 3363442..3363720 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CU079_RS16300
• Protein ID: WP_043016302.1
• Coordinates: 3363158..3363442 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU079_RS16285	3360849..3361733	+	885	WP_003833024.1	formate dehydrogenase N subunit beta	-
CU079_RS16290	3361726..3362382	+	657	WP_003020054.1	formate dehydrogenase-N subunit gamma	-
CU079_RS16295	3362512..3363114	+	603	WP_075146179.1	inorganic diphosphatase	-
CU079_RS16300	3363158..3363442	-	285	WP_043016302.1	HigA family addiction module antidote protein	Antitoxin
CU079_RS16305	3363442..3363720	-	279	WP_100194942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CU079_RS16310	3363941..3365656	-	1716	WP_100194375.1	ATP-binding cassette domain-containing protein	-
CU079_RS16315	3366121..3367023	+	903	WP_043016304.1	endonuclease/exonuclease/phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10666.23 Da
• Isoelectric Point: 9.8965

>T88625 WP_100194942.1 NZ_CP024819:c3363720-3363442 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRPAVIDAASKVTDINMPGYKLHPLVGERDGIWAISVSGNWRITFEFVN
GDAYIQDYEDYH

Nucleotide

• Length: 279 bp

>T88625 NZ_CP024819:c3363720-3363442 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGGCCTGCCGTTATTGATGCGGCAAGTAAGGTTACAGATATCAATATGCCCGGCTATAAGCTAC
ATCCTCTGGTGGGTGAGCGTGACGGCATATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGATGCGTACATACAGGACTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10357.07 Da
• Isoelectric Point: 8.8094

>AT88625 WP_043016302.1 NZ_CP024819:c3363442-3363158 [Citrobacter freundii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT88625 NZ_CP024819:c3363442-3363158 [Citrobacter freundii]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGACGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGGGCTATGGAAATTGCACCTTCAACGGCAAGTCGACTATTAACCGGGAAAGCGGCCCTGACGCCTGAGATGGCGA
TTAAACTCTCTGTGGTGATTGGCAGTTCACCCGAAATGTGGTTAAACCTGCAAAATACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGCCTGCGTAAGCTGGCAGTACAATGA