Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1131663..1132333 Replicon chromosome
Accession NZ_CP024819
Organism Citrobacter freundii strain CRCB-101

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CU079_RS05545 Protein ID WP_075145335.1
Coordinates 1131663..1131965 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CU079_RS05550 Protein ID WP_043018115.1
Coordinates 1131992..1132333 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU079_RS05525 1127208..1128731 - 1524 WP_075145337.1 sugar ABC transporter ATP-binding protein -
CU079_RS05535 1128846..1131110 + 2265 WP_100193849.1 hybrid sensor histidine kinase/response regulator -
CU079_RS05540 1131180..1131506 + 327 WP_047406937.1 hypothetical protein -
CU079_RS05545 1131663..1131965 + 303 WP_075145335.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU079_RS05550 1131992..1132333 + 342 WP_043018115.1 HigA family addiction module antidote protein Antitoxin
CU079_RS05555 1132386..1133144 - 759 WP_075145334.1 phosphonate metabolism protein PhnP -
CU079_RS05560 1133153..1133587 - 435 WP_043018117.1 aminoalkylphosphonate N-acetyltransferase -
CU079_RS05565 1133574..1134128 - 555 WP_043018118.1 ribose 1,5-bisphosphokinase -
CU079_RS05570 1134131..1135267 - 1137 WP_075145333.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
CU079_RS05575 1135264..1135950 - 687 WP_075146347.1 phosphonate C-P lyase system protein PhnL -
CU079_RS05580 1136095..1136853 - 759 WP_003826710.1 phosphonate C-P lyase system protein PhnK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-99)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11833.46 Da        Isoelectric Point: 9.9581

>T88621 WP_075145335.1 NZ_CP024819:1131663-1131965 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWINGKAEDVYLDPHIY

Download         Length: 303 bp

>T88621 NZ_CP024819:1131663-1131965 [Citrobacter freundii]
ATGTCTAAATCATTAAACATCAGAAGTTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCTGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATTATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAGTTGCAGGAATATTCTTCAATTAGAGTAAACAAGCAGTACCGA
TTAATATTCAAATGGATTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13075.88 Da        Isoelectric Point: 6.4763

>AT88621 WP_043018115.1 NZ_CP024819:1131992-1132333 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLELKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNGESKKVA

Download         Length: 342 bp

>AT88621 NZ_CP024819:1131992-1132333 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACTACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGAGCTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGTCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATGG
CGAGTCTAAAAAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References