Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 855763..856362 Replicon chromosome
Accession NZ_CP024819
Organism Citrobacter freundii strain CRCB-101

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CU079_RS04225 Protein ID WP_100193804.1
Coordinates 855763..856074 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CU079_RS04230 Protein ID WP_047407480.1
Coordinates 856075..856362 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU079_RS04190 851157..851756 + 600 WP_043018883.1 glucose-1-phosphatase -
CU079_RS04195 851750..852622 + 873 WP_043018884.1 virulence factor BrkB family protein -
CU079_RS04200 852619..853056 + 438 WP_100193801.1 D-tyrosyl-tRNA(Tyr) deacylase -
CU079_RS04205 853101..854042 + 942 WP_157787645.1 fatty acid biosynthesis protein FabY -
CU079_RS04210 854048..854335 - 288 WP_003825284.1 type II toxin-antitoxin system RelE/ParE family toxin -
CU079_RS04215 854328..854570 - 243 WP_003825282.1 type II toxin-antitoxin system ParD family antitoxin -
CU079_RS04220 854635..855543 - 909 WP_100193803.1 alpha/beta hydrolase -
CU079_RS04225 855763..856074 + 312 WP_100193804.1 toxin HigB-2 Toxin
CU079_RS04230 856075..856362 + 288 WP_047407480.1 helix-turn-helix domain-containing protein Antitoxin
CU079_RS04235 856406..857335 - 930 WP_043018887.1 formate dehydrogenase accessory protein FdhE -
CU079_RS04240 857332..857967 - 636 WP_043018888.1 formate dehydrogenase cytochrome b556 subunit -
CU079_RS04245 857964..858866 - 903 WP_003028691.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 838360..856362 18002


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(37-74)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12261.38 Da        Isoelectric Point: 10.0972

>T88619 WP_100193804.1 NZ_CP024819:855763-856074 [Citrobacter freundii]
MLFIETEIFTEDVKKLLDDDEYRRLQIFLTIQPDCGDLIQDTGGLRKVRWRARGKGKRGGVRIIYFHQIRKSQIRLLLIY
QKGIKDDLTPQEKVLLRMLNEGW

Download         Length: 312 bp

>T88619 NZ_CP024819:855763-856074 [Citrobacter freundii]
ATGCTATTCATAGAAACGGAAATCTTTACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGACTACAGAT
ATTCCTTACGATTCAGCCCGATTGTGGCGATCTGATACAAGATACAGGGGGCCTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGAAAACGTGGCGGTGTAAGGATTATCTACTTTCATCAAATCAGGAAATCACAAATTCGTTTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAGGAAAAAGTACTGCTGCGTATGTTGAATGAGGGGTGGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10819.40 Da        Isoelectric Point: 10.8907

>AT88619 WP_047407480.1 NZ_CP024819:856075-856362 [Citrobacter freundii]
MDKALFERLTQSMIQMNEIDEGTRQPSRTFHIDAMKIKEIRQSSGLSQTRFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPKHVIQALR

Download         Length: 288 bp

>AT88619 NZ_CP024819:856075-856362 [Citrobacter freundii]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGATTCAGATGAATGAGATCGATGAAGGAACGCGGCAGCCTTC
TCGTACCTTTCATATTGACGCCATGAAAATAAAGGAAATCCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGAAATTGGGAGCAGGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCCATTGCCAATGATCCAAAACATGTCATTCAGGCGCTAAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References