Detailed information of TA system TA088613

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 163085..163506
• Replicon: plasmid pCREC-629_1
• Accession: NZ_CP024816
• Organism: Escherichia coli strain CREC-629

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CU080_RS26760
• Protein ID: WP_001302184.1
• Coordinates: 163085..163243 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	163308..163506 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU080_RS26725	158149..158364	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
CU080_RS26730	158500..159147	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CU080_RS26735	159338..159721	-	384	WP_001063020.1	relaxosome protein TraM	-
CU080_RS26740	160091..160663	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CU080_RS26745	160959..161780	-	822	WP_001234465.1	DUF945 domain-containing protein	-
CU080_RS26750	161899..162186	-	288	WP_000107242.1	hypothetical protein	-
CU080_RS28400	162211..162417	-	207	WP_000275854.1	hypothetical protein	-
CU080_RS26760	163085..163243	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	163308..163506	-	199	NuclAT_0	-	Antitoxin
-	163308..163506	-	199	NuclAT_0	-	Antitoxin
-	163308..163506	-	199	NuclAT_0	-	Antitoxin
-	163308..163506	-	199	NuclAT_0	-	Antitoxin
CU080_RS26765	163475..163580	-	106	Protein_194	plasmid SOS inhibition protein A	-
CU080_RS26770	163735..164052	+	318	Protein_195	IS110 family transposase	-
CU080_RS26775	164341..164592	-	252	Protein_196	iron-containing alcohol dehydrogenase	-
CU080_RS26780	164677..165300	-	624	WP_000912556.1	potassium channel family protein	-
CU080_RS26785	165316..166009	-	694	Protein_198	IS1 family transposase	-
CU080_RS26795	166042..166620	+	579	WP_032147503.1	DUF2726 domain-containing protein	-
CU080_RS26800	167033..167386	-	354	WP_000005489.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(B) / erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17 / blaTEM-1B	-	1..176274	176274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T88613 WP_001302184.1 NZ_CP024816:c163243-163085 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88613 NZ_CP024816:c163243-163085 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT88613 NZ_CP024816:c163506-163308 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin