Detailed information of TA system TA088611

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 125668..125922
• Replicon: plasmid pCREC-629_1
• Accession: NZ_CP024816
• Organism: Escherichia coli strain CREC-629

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CU080_RS26530
• Protein ID: WP_015387399.1
• Coordinates: 125668..125874 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	125861..125922 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU080_RS26495	120915..121826	-	912	WP_000440183.1	carbamate kinase	-
CU080_RS26500	121837..123057	-	1221	WP_100183869.1	arginine deiminase	-
CU080_RS26505	123950..124432	+	483	Protein_145	VENN motif pre-toxin domain-containing protein	-
CU080_RS26510	124378..124824	-	447	Protein_146	incFII family plasmid replication initiator RepA	-
CU080_RS26515	124817..124891	-	75	WP_001365705.1	RepA leader peptide Tap	-
CU080_RS26525	125127..125384	-	258	WP_000083833.1	replication regulatory protein RepA	-
CU080_RS26530	125668..125874	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	125861..125922	+	62	NuclAT_1	-	Antitoxin
-	125861..125922	+	62	NuclAT_1	-	Antitoxin
-	125861..125922	+	62	NuclAT_1	-	Antitoxin
-	125861..125922	+	62	NuclAT_1	-	Antitoxin
CU080_RS26540	126061..126243	-	183	WP_000968309.1	hypothetical protein	-
CU080_RS26550	126344..127046	+	703	Protein_151	IS1 family transposase	-
CU080_RS26560	127347..127559	-	213	WP_005012601.1	hypothetical protein	-
CU080_RS26565	127692..128252	-	561	WP_000139363.1	fertility inhibition protein FinO	-
CU080_RS26570	128307..129053	-	747	WP_000205701.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(B) / erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17 / blaTEM-1B	-	1..176274	176274
	-	flank	IS/Tn	-	-	126660..127046	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T88611 WP_015387399.1 NZ_CP024816:c125874-125668 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T88611 NZ_CP024816:c125874-125668 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT88611 NZ_CP024816:125861-125922 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin