Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4552909..4553603 Replicon chromosome
Accession NZ_CP024815
Organism Escherichia coli strain CREC-629

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CU080_RS23730 Protein ID WP_001263489.1
Coordinates 4552909..4553307 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CU080_RS23735 Protein ID WP_000554758.1
Coordinates 4553310..4553603 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4548497..4548577 - 81 NuclAT_13 - -
- 4548497..4548577 - 81 NuclAT_13 - -
- 4548497..4548577 - 81 NuclAT_13 - -
- 4548497..4548577 - 81 NuclAT_13 - -
CU080_RS23700 4549173..4549631 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CU080_RS23705 4549892..4551349 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CU080_RS23710 4551406..4551927 - 522 Protein_4291 peptide chain release factor H -
CU080_RS23715 4551923..4552129 - 207 Protein_4292 RtcB family protein -
CU080_RS23725 4552447..4552899 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
CU080_RS23730 4552909..4553307 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CU080_RS23735 4553310..4553603 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CU080_RS23740 4553655..4554709 - 1055 Protein_4296 DNA polymerase IV -
CU080_RS23745 4554780..4555565 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CU080_RS23750 4555537..4557249 + 1713 Protein_4298 flagellar biosynthesis protein FlhA -
CU080_RS23755 4557473..4557970 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T88604 WP_001263489.1 NZ_CP024815:c4553307-4552909 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T88604 NZ_CP024815:c4553307-4552909 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT88604 WP_000554758.1 NZ_CP024815:c4553603-4553310 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT88604 NZ_CP024815:c4553603-4553310 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References