Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2540175..2540677 Replicon chromosome
Accession NZ_CP024815
Organism Escherichia coli strain CREC-629

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag CU080_RS12830 Protein ID WP_000767833.1
Coordinates 2540423..2540677 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CU080_RS12825 Protein ID WP_001259255.1
Coordinates 2540175..2540426 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU080_RS12795 2535353..2536420 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CU080_RS12800 2536420..2537490 - 1071 WP_000108939.1 histidinol-phosphate transaminase -
CU080_RS12805 2537493..2538791 - 1299 WP_049290931.1 histidinol dehydrogenase -
CU080_RS12810 2538797..2539696 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
CU080_RS12820 2539842..2539892 - 51 WP_001364200.1 his operon leader peptide -
CU080_RS12825 2540175..2540426 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CU080_RS12830 2540423..2540677 + 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CU080_RS12835 2540760..2541584 + 825 WP_000754758.1 SDR family oxidoreductase -
CU080_RS12840 2541630..2542559 + 930 WP_000803351.1 LysR family transcriptional regulator -
CU080_RS12845 2542774..2542836 + 63 WP_010723108.1 membrane protein YoeI -
CU080_RS12850 2542826..2544184 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CU080_RS12855 2544363..2545421 + 1059 WP_000492324.1 transport protein YeeE -
CU080_RS12860 2545435..2545662 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T88589 WP_000767833.1 NZ_CP024815:2540423-2540677 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88589 NZ_CP024815:2540423-2540677 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88589 WP_001259255.1 NZ_CP024815:2540175-2540426 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88589 NZ_CP024815:2540175-2540426 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References