Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4412939..4413666 Replicon chromosome
Accession NZ_CP024812
Organism Enterobacter sp. CRENT-193

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag CU081_RS22045 Protein ID WP_022650547.1
Coordinates 4412939..4413250 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag CU081_RS22050 Protein ID WP_022650546.1
Coordinates 4413247..4413666 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU081_RS22025 4409210..4409989 - 780 WP_003858848.1 acetolactate decarboxylase -
CU081_RS22030 4410095..4410976 + 882 WP_022650550.1 LysR family transcriptional regulator -
CU081_RS22035 4411259..4411714 + 456 WP_022650549.1 hypothetical protein -
CU081_RS26340 4411827..4412246 - 420 WP_032645895.1 hypothetical protein -
CU081_RS22045 4412939..4413250 + 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
CU081_RS22050 4413247..4413666 + 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
CU081_RS22055 4413990..4414424 + 435 WP_022650545.1 hypothetical protein -
CU081_RS22060 4414477..4415367 - 891 WP_022650544.1 oxidoreductase -
CU081_RS22065 4415473..4416594 + 1122 WP_003858868.1 glutamate--cysteine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T88578 WP_022650547.1 NZ_CP024812:4412939-4413250 [Enterobacter sp. CRENT-193]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T88578 NZ_CP024812:4412939-4413250 [Enterobacter sp. CRENT-193]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT88578 WP_022650546.1 NZ_CP024812:4413247-4413666 [Enterobacter sp. CRENT-193]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT88578 NZ_CP024812:4413247-4413666 [Enterobacter sp. CRENT-193]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References