Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1848365..1848867 Replicon chromosome
Accession NZ_CP024801
Organism Escherichia coli strain AMA1167

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CUC42_RS09350 Protein ID WP_000767829.1
Coordinates 1848613..1848867 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CUC42_RS09345 Protein ID WP_001259255.1
Coordinates 1848365..1848616 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC42_RS09315 1843542..1844609 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CUC42_RS09320 1844609..1845679 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
CUC42_RS09325 1845676..1846980 - 1305 WP_001317939.1 histidinol dehydrogenase -
CUC42_RS09330 1846986..1847885 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CUC42_RS09340 1848031..1848081 - 51 WP_001364200.1 his operon leader peptide -
CUC42_RS09345 1848365..1848616 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CUC42_RS09350 1848613..1848867 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CUC42_RS09355 1848950..1849774 + 825 WP_000754758.1 SDR family oxidoreductase -
CUC42_RS09360 1849820..1850749 + 930 WP_000803351.1 LysR family transcriptional regulator -
CUC42_RS09365 1850964..1851026 + 63 WP_010723108.1 membrane protein YoeI -
CUC42_RS09370 1851016..1852374 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CUC42_RS09375 1852553..1853611 + 1059 WP_000492346.1 transport protein YeeE -
CUC42_RS09380 1853625..1853852 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88532 WP_000767829.1 NZ_CP024801:1848613-1848867 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88532 NZ_CP024801:1848613-1848867 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88532 WP_001259255.1 NZ_CP024801:1848365-1848616 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88532 NZ_CP024801:1848365-1848616 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References