Detailed information of TA system TA088485

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77258..77684
• Replicon: plasmid p1NQ3
• Accession: NZ_CP024721
• Organism: Escherichia coli isolate NQ3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CUB98_RS27505
• Protein ID: WP_001312861.1
• Coordinates: 77258..77416 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	77460..77684 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB98_RS27455	72296..72523	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CUB98_RS27460	72617..73303	-	687	WP_000332487.1	PAS domain-containing protein	-
CUB98_RS27465	73497..73880	-	384	WP_001151564.1	relaxosome protein TraM	-
CUB98_RS27470	74166..74813	+	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
CUB98_RS27475	75110..75931	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CUB98_RS27480	76052..76339	-	288	WP_099996262.1	hypothetical protein	-
CUB98_RS27505	77258..77416	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77460..77684	-	225	NuclAT_0	-	Antitoxin
-	77460..77684	-	225	NuclAT_0	-	Antitoxin
-	77460..77684	-	225	NuclAT_0	-	Antitoxin
-	77460..77684	-	225	NuclAT_0	-	Antitoxin
CUB98_RS28080	77496..77684	+	189	WP_001299721.1	hypothetical protein	-
CUB98_RS27510	77696..78415	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CUB98_RS27515	78412..78846	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
CUB98_RS27520	78901..80859	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
CUB98_RS27525	80918..81151	-	234	WP_000006004.1	DUF905 domain-containing protein	-
CUB98_RS27530	81207..81734	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1	-	1..111776	111776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88485 WP_001312861.1 NZ_CP024721:c77416-77258 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88485 NZ_CP024721:c77416-77258 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT88485 NZ_CP024721:c77684-77460 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin