Detailed information of TA system TA088482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4564463..4565065
• Replicon: chromosome
• Accession: NZ_CP024720
• Organism: Escherichia coli isolate NQ3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CUB98_RS23865
• Protein ID: WP_000897302.1
• Coordinates: 4564754..4565065 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUB98_RS23860
• Protein ID: WP_000356397.1
• Coordinates: 4564463..4564753 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB98_RS23820	4559770..4560699	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
CUB98_RS23825	4560881..4561123	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
CUB98_RS23830	4561413..4562261	+	849	WP_001038650.1	hypothetical protein	-
CUB98_RS23835	4562577..4563026	+	450	WP_032140890.1	hypothetical protein	-
CUB98_RS23840	4563211..4563429	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
CUB98_RS23850	4563826..4564104	-	279	WP_001296612.1	hypothetical protein	-
CUB98_RS23860	4564463..4564753	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CUB98_RS23865	4564754..4565065	-	312	WP_000897302.1	hypothetical protein	Toxin
CUB98_RS23870	4565294..4566202	+	909	WP_001385591.1	alpha/beta hydrolase	-
CUB98_RS23875	4566266..4567207	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CUB98_RS23880	4567252..4567689	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CUB98_RS23885	4567686..4568558	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CUB98_RS23890	4568552..4569151	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T88482 WP_000897302.1 NZ_CP024720:c4565065-4564754 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T88482 NZ_CP024720:c4565065-4564754 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT88482 WP_000356397.1 NZ_CP024720:c4564753-4564463 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT88482 NZ_CP024720:c4564753-4564463 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416