Detailed information of TA system TA088443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4444619..4445121
• Replicon: chromosome
• Accession: NZ_CP024717
• Organism: Escherichia coli strain LS4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: CUB99_RS22910
• Protein ID: WP_000767819.1
• Coordinates: 4444867..4445121 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: CUB99_RS22905
• Protein ID: WP_001259253.1
• Coordinates: 4444619..4444870 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB99_RS22870	4439797..4440864	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
CUB99_RS22875	4440864..4441934	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
CUB99_RS22880	4441931..4443235	-	1305	WP_001296211.1	histidinol dehydrogenase	-
CUB99_RS22885	4443241..4444140	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
CUB99_RS22895	4444286..4444336	-	51	WP_001364200.1	his operon leader peptide	-
CUB99_RS22905	4444619..4444870	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
CUB99_RS22910	4444867..4445121	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
CUB99_RS22915	4445204..4446028	+	825	WP_000754737.1	SDR family oxidoreductase	-
CUB99_RS22920	4446074..4447003	+	930	WP_000803366.1	LysR family transcriptional regulator	-
CUB99_RS22925	4447218..4447280	+	63	WP_010723108.1	membrane protein YoeI	-
CUB99_RS22930	4447270..4448628	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
CUB99_RS22940	4448845..4449303	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4448845..4449303	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T88443 WP_000767819.1 NZ_CP024717:4444867-4445121 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T88443 NZ_CP024717:4444867-4445121 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT88443 WP_001259253.1 NZ_CP024717:4444619-4444870 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT88443 NZ_CP024717:4444619-4444870 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3