Detailed information of TA system TA088437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2318864..2319466
• Replicon: chromosome
• Accession: NZ_CP024717
• Organism: Escherichia coli strain LS4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CUB99_RS12290
• Protein ID: WP_000897302.1
• Coordinates: 2319155..2319466 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUB99_RS12285
• Protein ID: WP_000356397.1
• Coordinates: 2318864..2319154 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB99_RS12245	2314171..2315100	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
CUB99_RS12250	2315282..2315524	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
CUB99_RS12255	2315814..2316662	+	849	WP_001038650.1	hypothetical protein	-
CUB99_RS12260	2316978..2317427	+	450	WP_032140890.1	hypothetical protein	-
CUB99_RS12265	2317612..2317830	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
CUB99_RS12275	2318227..2318505	-	279	WP_001296612.1	hypothetical protein	-
CUB99_RS12285	2318864..2319154	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CUB99_RS12290	2319155..2319466	-	312	WP_000897302.1	hypothetical protein	Toxin
CUB99_RS12295	2319695..2320603	+	909	WP_001385591.1	alpha/beta hydrolase	-
CUB99_RS12300	2320667..2321608	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CUB99_RS12305	2321653..2322090	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CUB99_RS12310	2322087..2322959	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CUB99_RS12315	2322953..2323552	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T88437 WP_000897302.1 NZ_CP024717:c2319466-2319155 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T88437 NZ_CP024717:c2319466-2319155 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT88437 WP_000356397.1 NZ_CP024717:c2319154-2318864 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT88437 NZ_CP024717:c2319154-2318864 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416