88428

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6278999..6279592 Replicon chromosome
Accession NZ_CP024712
Organism Pseudomonas syringae pv. actinidiae strain MAFF212063

Toxin (Protein)


Gene name graT Uniprot ID A0A2G7PD43
Locus tag CT122_RS29915 Protein ID WP_003377943.1
Coordinates 6279314..6279592 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88B19
Locus tag CT122_RS29910 Protein ID WP_005739893.1
Coordinates 6278999..6279304 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CT122_RS29870 6274688..6275380 + 693 WP_003377932.1 HAD family hydrolase -
CT122_RS29875 6275455..6275787 - 333 WP_003377934.1 hypothetical protein -
CT122_RS29880 6275886..6276311 - 426 WP_003377936.1 secondary thiamine-phosphate synthase enzyme YjbQ -
CT122_RS29890 6276554..6277891 - 1338 WP_003377937.1 ammonium transporter -
CT122_RS29895 6277926..6278264 - 339 WP_002555808.1 P-II family nitrogen regulator -
CT122_RS29905 6278684..6278965 + 282 WP_003377939.1 accessory factor UbiK family protein -
CT122_RS29910 6278999..6279304 - 306 WP_005739893.1 HigA family addiction module antidote protein Antitoxin
CT122_RS29915 6279314..6279592 - 279 WP_003377943.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CT122_RS29920 6279763..6280677 - 915 WP_019332092.1 LysR family transcriptional regulator -
CT122_RS29925 6281162..6282682 + 1521 WP_020308287.1 amino acid ABC transporter permease/ATP-binding protein -
CT122_RS29930 6282707..6283579 + 873 WP_005613618.1 phytanoyl-CoA dioxygenase family protein -
CT122_RS29935 6283607..6284440 + 834 WP_005739898.1 transporter substrate-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10512.18 Da        Isoelectric Point: 9.1490

>T88428 WP_003377943.1 NZ_CP024712:c6279592-6279314 [Pseudomonas syringae pv. actinidiae]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Download         Length: 279 bp

>T88428 NZ_CP024712:c6279592-6279314 [Pseudomonas syringae pv. actinidiae]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGTGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGATGTAGAAATTGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11158.01 Da        Isoelectric Point: 7.7509

>AT88428 WP_005739893.1 NZ_CP024712:c6279304-6278999 [Pseudomonas syringae pv. actinidiae]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Download         Length: 306 bp

>AT88428 NZ_CP024712:c6279304-6278999 [Pseudomonas syringae pv. actinidiae]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGAGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7PD43

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LYY6

References