Detailed information of TA system TA088399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2646560..2647254
• Replicon: chromosome
• Accession: NZ_CP024710
• Organism: Dickeya solani strain IFB0223

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: CTB91_RS11830
• Protein ID: WP_026594479.1
• Coordinates: 2646560..2646958 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: A0A2K8VUD1
• Locus tag: CTB91_RS11835
• Protein ID: WP_022633740.1
• Coordinates: 2646961..2647254 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTB91_RS11800	2641740..2642774	-	1035	WP_022633732.1	harpin HrpZ family protein	-
CTB91_RS11805	2642935..2643345	-	411	WP_022633733.1	hypothetical protein	-
CTB91_RS11810	2643342..2643530	-	189	WP_022633734.1	hypothetical protein	-
CTB91_RS11815	2643565..2645634	-	2070	WP_023637836.1	type III secretion system outer membrane ring subunit SctC	-
CTB91_RS11820	2645627..2646061	-	435	WP_022633737.1	hypothetical protein	-
CTB91_RS11825	2646048..2646275	-	228	WP_022633738.1	type III secretion protein HrpF	-
CTB91_RS11830	2646560..2646958	-	399	WP_026594479.1	type II toxin-antitoxin system YafO family toxin	Toxin
CTB91_RS11835	2646961..2647254	-	294	WP_022633740.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CTB91_RS11840	2647472..2648521	-	1050	WP_022633741.1	hypothetical protein	-
CTB91_RS11845	2649061..2650221	+	1161	WP_022633742.1	alpha/beta fold hydrolase	-
CTB91_RS11850	2650232..2651545	-	1314	WP_022633743.1	lytic murein transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2646048..2655976	9928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15230.56 Da
• Isoelectric Point: 8.4931

>T88399 WP_026594479.1 NZ_CP024710:c2646958-2646560 [Dickeya solani]
IRIFKSTLIRQQLSQQELDDLVADFHAYKQNGVLPDTFGRDAPYDDDRTYPLVKAEQVAHIHLADADAPFPRFLRQFKRT
SDRAHLVYCQGAMDPEACLLIIILKPEAHKMARNNNNMHKIGMMAAAFRMKY

Nucleotide

• Length: 399 bp

>T88399 NZ_CP024710:c2646958-2646560 [Dickeya solani]
ATACGGATTTTCAAGTCAACGCTGATCCGTCAACAATTGAGCCAGCAAGAGCTTGATGATTTGGTCGCGGATTTTCACGC
CTATAAGCAGAATGGGGTACTACCGGACACATTTGGGCGTGACGCGCCTTATGATGATGACCGAACCTATCCATTAGTAA
AAGCAGAGCAGGTGGCTCACATCCACCTTGCCGATGCGGATGCGCCTTTTCCCAGGTTCTTGCGCCAGTTCAAGAGAACC
AGCGATCGGGCCCATTTGGTCTACTGCCAGGGAGCGATGGATCCCGAGGCCTGCCTGTTAATTATTATTTTAAAACCCGA
AGCCCATAAAATGGCACGCAACAATAACAACATGCACAAGATCGGGATGATGGCCGCTGCGTTCAGGATGAAATATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10934.48 Da
• Isoelectric Point: 5.2082

>AT88399 WP_022633740.1 NZ_CP024710:c2647254-2646961 [Dickeya solani]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEAKQETVHTVARFRPTAERLRALADSG
QKLLNDASGKDLTEFTE

Nucleotide

• Length: 294 bp

>AT88399 NZ_CP024710:c2647254-2646961 [Dickeya solani]
ATGGAGCGTATTCTTGCTGAAAAATCTATAAATATTACAGAGTTAAGGAAAAATCCGGCTAAGTATTTTATTGATGAGCC
TGTTGCCGTGCTTTCTAACAACCGGCCCGCCGGTTACATGGTGAGTGCCAAAGTCTTTGAAGAACTGATCGATCTGCTAG
AGGCGAAGCAGGAAACGGTTCATACCGTAGCGCGCTTTAGGCCAACGGCCGAACGTTTACGAGCCCTTGCAGATAGCGGT
CAGAAACTGCTGAATGATGCCTCTGGCAAGGATCTGACTGAGTTCACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8VUD1