Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4341530..4342178 Replicon chromosome
Accession NZ_CP024679
Organism Citrobacter freundii strain UMH15

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag CUC47_RS21655 Protein ID WP_016151211.1
Coordinates 4341816..4342178 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B5QFP8
Locus tag CUC47_RS21650 Protein ID WP_003844470.1
Coordinates 4341530..4341829 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC47_RS21630 4337852..4338268 - 417 WP_003023951.1 GNAT family N-acetyltransferase -
CUC47_RS21635 4338399..4338692 + 294 WP_008323809.1 hypothetical protein -
CUC47_RS21640 4338787..4340169 + 1383 WP_106902615.1 glycoside hydrolase family 1 protein -
CUC47_RS21645 4340218..4341411 - 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
CUC47_RS21650 4341530..4341829 - 300 WP_003844470.1 helix-turn-helix transcriptional regulator Antitoxin
CUC47_RS21655 4341816..4342178 - 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUC47_RS21660 4342279..4342731 - 453 WP_121540570.1 DUF1198 family protein -
CUC47_RS21665 4342857..4344248 - 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
CUC47_RS21670 4344391..4345719 - 1329 WP_003023927.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T88315 WP_016151211.1 NZ_CP024679:c4342178-4341816 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T88315 NZ_CP024679:c4342178-4341816 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11032.85 Da        Isoelectric Point: 8.5047

>AT88315 WP_003844470.1 NZ_CP024679:c4341829-4341530 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQSDNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT88315 NZ_CP024679:c4341829-4341530 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAACCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGTGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGTCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5QFP8

References