Detailed information of TA system TA088298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4827026..4827696
• Replicon: chromosome
• Accession: NZ_CP024677
• Organism: Citrobacter freundii strain UMH16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: CUC48_RS23950
• Protein ID: WP_046671374.1
• Coordinates: 4827026..4827328 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUC48_RS23955
• Protein ID: WP_046671375.1
• Coordinates: 4827355..4827696 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC48_RS23930	4822569..4824092	-	1524	WP_086529673.1	sugar ABC transporter ATP-binding protein	-
CUC48_RS23940	4824207..4826471	+	2265	WP_086529672.1	hybrid sensor histidine kinase/response regulator	-
CUC48_RS23945	4826541..4826870	+	330	WP_003031807.1	hypothetical protein	-
CUC48_RS23950	4827026..4827328	+	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUC48_RS23955	4827355..4827696	+	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
CUC48_RS23960	4827749..4828507	-	759	WP_086529671.1	phosphonate metabolism protein PhnP	-
CUC48_RS23965	4828516..4828950	-	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
CUC48_RS23970	4828937..4829491	-	555	WP_003841102.1	ribose 1,5-bisphosphokinase	-
CUC48_RS23975	4829494..4830630	-	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
CUC48_RS23980	4830627..4831307	-	681	WP_046671387.1	phosphonate C-P lyase system protein PhnL	-
CUC48_RS23985	4831397..4832155	-	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T88298 WP_046671374.1 NZ_CP024677:4827026-4827328 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T88298 NZ_CP024677:4827026-4827328 [Citrobacter freundii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT88298 WP_046671375.1 NZ_CP024677:4827355-4827696 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT88298 NZ_CP024677:4827355-4827696 [Citrobacter freundii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6