88294

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4067114..4067780 Replicon chromosome
Accession NZ_CP024677
Organism Citrobacter freundii strain UMH16

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag CUC48_RS20315 Protein ID WP_003847996.1
Coordinates 4067114..4067431 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag CUC48_RS20320 Protein ID WP_003837894.1
Coordinates 4067484..4067780 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC48_RS20300 4062284..4063162 + 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -
CUC48_RS20305 4063272..4064990 - 1719 WP_086538628.1 DUF4153 domain-containing protein -
CUC48_RS20310 4065369..4066991 + 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
CUC48_RS20315 4067114..4067431 + 318 WP_003847996.1 hypothetical protein Toxin
CUC48_RS20320 4067484..4067780 + 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
CUC48_RS20325 4067837..4069189 - 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
CUC48_RS20330 4069186..4069905 - 720 WP_001157751.1 two-component system response regulator OmpR -
CUC48_RS20335 4070131..4070604 + 474 WP_003023524.1 transcription elongation factor GreB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T88294 WP_003847996.1 NZ_CP024677:4067114-4067431 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T88294 NZ_CP024677:4067114-4067431 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT88294 WP_003837894.1 NZ_CP024677:4067484-4067780 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT88294 NZ_CP024677:4067484-4067780 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References