Detailed information of TA system TA088276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4315789..4316459
• Replicon: chromosome
• Accession: NZ_CP024676
• Organism: Citrobacter pasteurii strain UMH17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: CUC49_RS21035
• Protein ID: WP_046671374.1
• Coordinates: 4315789..4316091 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUC49_RS21040
• Protein ID: WP_048213890.1
• Coordinates: 4316118..4316459 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC49_RS21015	4311332..4312855	-	1524	WP_121585345.1	sugar ABC transporter ATP-binding protein	-
CUC49_RS21025	4312970..4315234	+	2265	WP_121585346.1	hybrid sensor histidine kinase/response regulator	-
CUC49_RS21030	4315304..4315633	+	330	WP_048213892.1	hypothetical protein	-
CUC49_RS21035	4315789..4316091	+	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CUC49_RS21040	4316118..4316459	+	342	WP_048213890.1	HigA family addiction module antidote protein	Antitoxin
CUC49_RS21045	4316512..4317270	-	759	WP_121585347.1	phosphonate metabolism protein PhnP	-
CUC49_RS21050	4317279..4317713	-	435	WP_048213888.1	aminoalkylphosphonate N-acetyltransferase	-
CUC49_RS21055	4317700..4318254	-	555	WP_003841102.1	ribose 1,5-bisphosphokinase	-
CUC49_RS21060	4318257..4319393	-	1137	WP_121585348.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
CUC49_RS21065	4319390..4320070	-	681	WP_121585349.1	phosphonate C-P lyase system protein PhnL	-
CUC49_RS21070	4320080..4320838	-	759	WP_048213884.1	phosphonate C-P lyase system protein PhnK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T88276 WP_046671374.1 NZ_CP024676:4315789-4316091 [Citrobacter pasteurii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T88276 NZ_CP024676:4315789-4316091 [Citrobacter pasteurii]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13089.90 Da
• Isoelectric Point: 6.4763

>AT88276 WP_048213890.1 NZ_CP024676:4316118-4316459 [Citrobacter pasteurii]
MKQATRKPTTVGDILQYEYLEPLELKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT88276 NZ_CP024676:4316118-4316459 [Citrobacter pasteurii]
ATGAAACAGGCAACCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGAACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6