Detailed information of TA system TA088273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4060161..4060915
• Replicon: chromosome
• Accession: NZ_CP024676
• Organism: Citrobacter pasteurii strain UMH17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CUC49_RS19820
• Protein ID: WP_121585274.1
• Coordinates: 4060604..4060915 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUC49_RS19815
• Protein ID: WP_040233078.1
• Coordinates: 4060161..4060607 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUC49_RS19785	4055425..4056321	+	897	WP_121585272.1	sugar kinase	-
CUC49_RS19790	4056355..4057158	+	804	WP_003847907.1	DeoR/GlpR family DNA-binding transcription regulator	-
CUC49_RS19795	4057264..4057863	+	600	WP_048214033.1	glucose-1-phosphatase	-
CUC49_RS19800	4057857..4058729	+	873	WP_121585273.1	virulence factor BrkB family protein	-
CUC49_RS19805	4058726..4059163	+	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CUC49_RS19810	4059208..4060149	+	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
CUC49_RS19815	4060161..4060607	-	447	WP_040233078.1	helix-turn-helix domain-containing protein	Antitoxin
CUC49_RS19820	4060604..4060915	-	312	WP_121585274.1	type II toxin-antitoxin system HigB family toxin	Toxin
CUC49_RS19825	4061001..4061930	-	930	WP_121585275.1	alpha/beta hydrolase	-
CUC49_RS19830	4062135..4062377	+	243	WP_005121822.1	ribbon-helix-helix domain-containing protein	-
CUC49_RS19835	4062377..4062751	+	375	WP_005121821.1	type II toxin-antitoxin system VapC family toxin	-
CUC49_RS19840	4062788..4063717	-	930	WP_061066954.1	formate dehydrogenase accessory protein FdhE	-
CUC49_RS19845	4063714..4064349	-	636	WP_043018888.1	formate dehydrogenase cytochrome b556 subunit	-
CUC49_RS19850	4064346..4065248	-	903	WP_003028691.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12453.38 Da
• Isoelectric Point: 8.4457

>T88273 WP_121585274.1 NZ_CP024676:c4060915-4060604 [Citrobacter pasteurii]
VHVISRKPFNEGMLMYPNHGLALSELLNVLEKKTFHHPEEMKQYIPSLDNFKYRNKWWVIDVSGNNLRLIAYIDFRLHKV
FVEHIVLHAEYDKLTEYYRGHKE

Nucleotide

• Length: 312 bp

>T88273 NZ_CP024676:c4060915-4060604 [Citrobacter pasteurii]
GTGCATGTTATCAGCCGAAAACCATTCAATGAGGGTATGCTCATGTATCCAAACCATGGGCTTGCTCTCTCTGAGTTGCT
GAATGTTTTGGAGAAAAAAACATTCCATCACCCTGAAGAGATGAAACAGTATATTCCTTCTCTTGATAATTTTAAGTACA
GGAATAAATGGTGGGTAATTGATGTTTCTGGTAACAACCTGAGACTCATTGCCTACATAGATTTCAGGTTACATAAGGTA
TTTGTGGAGCATATCGTTCTCCACGCCGAATATGACAAACTGACAGAATATTATCGGGGCCATAAAGAATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16685.08 Da
• Isoelectric Point: 7.0634

>AT88273 WP_040233078.1 NZ_CP024676:c4060607-4060161 [Citrobacter pasteurii]
MRSHRHMDAASAKKIVDTFSCAVKTIPLLGEERNETEYRRALALVEFLVDHDDLENPLFELLSARISEYEKHAPEFRALN
QHLKQIPSGVSVLRTLMDQYGLKATDLANELGSKSNVSNILNGRRALTVSHIKALAERFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT88273 NZ_CP024676:c4060607-4060161 [Citrobacter pasteurii]
ATGAGATCTCATCGTCATATGGACGCAGCCAGCGCAAAAAAGATCGTTGATACCTTCAGTTGTGCTGTAAAAACGATCCC
TCTTTTGGGGGAAGAACGCAATGAAACGGAGTATCGAAGGGCACTAGCGCTAGTAGAGTTTCTGGTTGACCACGACGATC
TTGAAAACCCACTATTTGAATTACTTAGTGCCCGAATCAGTGAATACGAAAAACATGCACCAGAATTCAGAGCGCTCAAT
CAACACCTGAAACAAATCCCCTCTGGCGTTTCCGTATTGCGTACGCTCATGGATCAGTACGGATTAAAAGCCACCGACCT
CGCCAACGAGCTTGGTTCTAAATCGAACGTCAGCAACATCCTAAATGGCCGTAGAGCACTGACTGTAAGCCATATTAAAG
CGCTTGCAGAACGCTTTAAACTTCCGGCAGATGCATTTATCGAGTAG