Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4687184..4687847 Replicon chromosome
Accession NZ_CP024675
Organism Citrobacter werkmanii strain UMH18

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3X092
Locus tag CUC50_RS22775 Protein ID WP_038636359.1
Coordinates 4687184..4687486 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3X2H4
Locus tag CUC50_RS22780 Protein ID WP_038636361.1
Coordinates 4687506..4687847 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC50_RS22755 4682727..4684250 - 1524 WP_038636350.1 sugar ABC transporter ATP-binding protein -
CUC50_RS22765 4684365..4686629 + 2265 WP_121529850.1 hybrid sensor histidine kinase/response regulator -
CUC50_RS22770 4686699..4687028 + 330 WP_069325126.1 hypothetical protein -
CUC50_RS22775 4687184..4687486 + 303 WP_038636359.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUC50_RS22780 4687506..4687847 + 342 WP_038636361.1 HigA family addiction module antidote protein Antitoxin
CUC50_RS22785 4687899..4688657 - 759 WP_121529851.1 phosphonate metabolism protein PhnP -
CUC50_RS22790 4688666..4689100 - 435 WP_069325128.1 aminoalkylphosphonate N-acetyltransferase -
CUC50_RS22795 4689087..4689641 - 555 WP_038636371.1 ribose 1,5-bisphosphokinase -
CUC50_RS22800 4689644..4690780 - 1137 WP_085049652.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
CUC50_RS22805 4690777..4691457 - 681 WP_121529852.1 phosphonate C-P lyase system protein PhnL -
CUC50_RS22810 4691565..4692323 - 759 WP_038636380.1 phosphonate C-P lyase system protein PhnK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11856.50 Da        Isoelectric Point: 9.9581

>T88261 WP_038636359.1 NZ_CP024675:4687184-4687486 [Citrobacter werkmanii]
MSKSLNIRNFRDTWLEDFFERATPHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Download         Length: 303 bp

>T88261 NZ_CP024675:4687184-4687486 [Citrobacter werkmanii]
ATGTCTAAATCATTAAATATCAGAAATTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCAACACCGCACAGAAA
AATACCCGCGGATATTCACACCGCATTAGCCAGAAAGCTGGATATCATCAATGCGGCGGTTTCGCATCGGGATTTGCGCT
CGCCGCCAGGGAATAGATATGAAGAGTTAACCGGAAAGTTACAGGAATATTCTTCAATAAGAGTCAATAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGACCCACACATCTACTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13063.93 Da        Isoelectric Point: 7.2038

>AT88261 WP_038636361.1 NZ_CP024675:4687506-4687847 [Citrobacter werkmanii]
MKQATRKPTTVGDVLQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIMSVKDFVAQRNVATKKVA

Download         Length: 342 bp

>AT88261 NZ_CP024675:4687506-4687847 [Citrobacter werkmanii]
ATGAAACAGGCAACCAGAAAACCAACTACTGTGGGTGATGTCCTGCAATATGAATACCTGGAGCCGCTCGATCTGAAAAT
TAACGATCTAGCTGAAATACTGCATGTTCACCGTAATACGGTGAGCGCACTGGTCAATAATAATCGCAAATTAACAACGG
ATATGGCATTTCGCCTGGCGAAAGCGTTTGATACCTCCGTTGATTTCTGGCGTAACCTACAGTCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGTATTATGTCTGTTAAAGACTTTGTCGCGCAGCGAAATGT
CGCCACTAAGAAAGTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X092

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3X2H4

References