Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4135285..4135933 Replicon chromosome
Accession NZ_CP024672
Organism Citrobacter freundii strain HM38

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag CUC52_RS20420 Protein ID WP_016151211.1
Coordinates 4135571..4135933 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag CUC52_RS20415 Protein ID WP_003840660.1
Coordinates 4135285..4135584 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUC52_RS20395 4131606..4132022 - 417 WP_048218859.1 GNAT family N-acetyltransferase -
CUC52_RS20400 4132153..4132446 + 294 WP_003840665.1 hypothetical protein -
CUC52_RS20405 4132541..4133923 + 1383 WP_060855164.1 glycoside hydrolase family 1 protein -
CUC52_RS20410 4133973..4135166 - 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
CUC52_RS20415 4135285..4135584 - 300 WP_003840660.1 helix-turn-helix transcriptional regulator Antitoxin
CUC52_RS20420 4135571..4135933 - 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CUC52_RS20425 4136034..4136486 - 453 WP_003023933.1 DUF1198 family protein -
CUC52_RS20430 4136612..4138003 - 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
CUC52_RS20435 4138146..4139474 - 1329 WP_003023927.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T88227 WP_016151211.1 NZ_CP024672:c4135933-4135571 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T88227 NZ_CP024672:c4135933-4135571 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT88227 WP_003840660.1 NZ_CP024672:c4135584-4135285 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT88227 NZ_CP024672:c4135584-4135285 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References