Detailed information of TA system TA088202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3394244..3394971
• Replicon: chromosome
• Accession: NZ_CP024659
• Organism: Escherichia coli strain RM9872-C1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CUB84_RS16805
• Protein ID: WP_000547563.1
• Coordinates: 3394660..3394971 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CUB84_RS16800
• Protein ID: WP_000126294.1
• Coordinates: 3394244..3394663 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB84_RS16780	3389293..3389820	-	528	WP_001078777.1	electron transport protein HydN	-
CUB84_RS16785	3389969..3390982	-	1014	WP_024221754.1	DNA-binding transcriptional regulator AscG	-
CUB84_RS16790	3391240..3392697	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CUB84_RS16795	3392706..3394130	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
CUB84_RS16800	3394244..3394663	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CUB84_RS16805	3394660..3394971	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CUB84_RS16810	3395133..3395888	-	756	WP_001400787.1	hypothetical protein	-
CUB84_RS16815	3395931..3396398	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
CUB84_RS16820	3396391..3396801	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
CUB84_RS16825	3396798..3397565	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
CUB84_RS16830	3397565..3398107	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CUB84_RS16835	3398117..3399826	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T88202 WP_000547563.1 NZ_CP024659:c3394971-3394660 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T88202 NZ_CP024659:c3394971-3394660 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT88202 WP_000126294.1 NZ_CP024659:c3394663-3394244 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT88202 NZ_CP024659:c3394663-3394244 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22