Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2633116..2633618 Replicon chromosome
Accession NZ_CP024659
Organism Escherichia coli strain RM9872-C1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CUB84_RS13285 Protein ID WP_000767829.1
Coordinates 2633116..2633370 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CUB84_RS13290 Protein ID WP_001259255.1
Coordinates 2633367..2633618 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CUB84_RS13255 2628131..2628358 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CUB84_RS13260 2628372..2629430 - 1059 WP_000492332.1 transport protein YeeE -
CUB84_RS13265 2629609..2630967 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CUB84_RS13270 2630957..2631019 - 63 WP_010723108.1 membrane protein YoeI -
CUB84_RS13275 2631234..2632163 - 930 WP_000803351.1 LysR family transcriptional regulator -
CUB84_RS13280 2632209..2633033 - 825 WP_000754755.1 SDR family oxidoreductase -
CUB84_RS13285 2633116..2633370 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CUB84_RS13290 2633367..2633618 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CUB84_RS13295 2633902..2633952 + 51 WP_001364200.1 his operon leader peptide -
CUB84_RS13300 2634098..2634997 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CUB84_RS13305 2635003..2636307 + 1305 WP_001400693.1 histidinol dehydrogenase -
CUB84_RS13310 2636304..2637374 + 1071 WP_001099213.1 histidinol-phosphate transaminase -
CUB84_RS13315 2637374..2638441 + 1068 WP_000080083.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88201 WP_000767829.1 NZ_CP024659:c2633370-2633116 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88201 NZ_CP024659:c2633370-2633116 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88201 WP_001259255.1 NZ_CP024659:c2633618-2633367 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88201 NZ_CP024659:c2633618-2633367 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References