Detailed information of TA system TA088181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50479..50749
• Replicon: plasmid pBH100-1
• Accession: NZ_CP024652
• Organism: Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CDW44_RS26230
• Protein ID: WP_001312861.1
• Coordinates: 50591..50749 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	50479..50542 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDW44_RS26195	46100..46693	-	594	WP_000428546.1	tetracyline resistance-associated transcriptional repressor TetC	-
CDW44_RS26200	46826..47197	+	372	WP_035689358.1	helix-turn-helix domain-containing protein	-
CDW44_RS26205	47207..48415	-	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
CDW44_RS26215	48525..49106	+	582	Protein_54	hypothetical protein	-
CDW44_RS26220	49161..49595	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
CDW44_RS26225	49592..50311	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	50323..50547	+	225	NuclAT_0	-	-
-	50323..50547	+	225	NuclAT_0	-	-
-	50323..50547	+	225	NuclAT_0	-	-
-	50323..50547	+	225	NuclAT_0	-	-
CDW44_RS27025	50332..50511	-	180	WP_001309233.1	hypothetical protein	-
-	50479..50542	-	64	-	-	Antitoxin
CDW44_RS26230	50591..50749	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CDW44_RS27140	50987..51364	-	378	Protein_59	hypothetical protein	-
CDW44_RS26250	51663..51959	+	297	WP_001272251.1	hypothetical protein	-
CDW44_RS26255	52070..52891	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CDW44_RS26260	53186..53788	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
CDW44_RS26270	54111..54494	+	384	WP_001354030.1	relaxosome protein TraM	-
CDW44_RS26275	54688..55359	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
CDW44_RS26280	55496..55723	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / catA1 / aph(3')-Ia / tet(B) / blaTEM-1A	-	1..107274	107274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88181 WP_001312861.1 NZ_CP024652:50591-50749 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88181 NZ_CP024652:50591-50749 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT88181 NZ_CP024652:c50542-50479 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin