Detailed information of TA system TA088177

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 4961573..4961985
• Replicon: chromosome
• Accession: NZ_CP024650
• Organism: Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: CDW44_RS25135
• Protein ID: WP_000132614.1
• Coordinates: 4961573..4961914 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	4961909..4961985 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDW44_RS25115	4956642..4957922	-	1281	WP_001298033.1	DUF445 domain-containing protein	-
CDW44_RS25120	4958107..4959027	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
CDW44_RS25125	4959255..4959335	+	81	WP_020233658.1	hypothetical protein	-
CDW44_RS25130	4959433..4961526	+	2094	WP_000648237.1	DUF262 domain-containing protein	-
CDW44_RS25135	4961573..4961914	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	4961909..4961985	+	77	NuclAT_6	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_6	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_6	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_6	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_7	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_7	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_7	-	Antitoxin
-	4961909..4961985	+	77	NuclAT_7	-	Antitoxin
CDW44_RS25140	4962135..4963841	-	1707	WP_001100194.1	restriction endonuclease subunit S	-
CDW44_RS25145	4963841..4965310	-	1470	WP_001540814.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T88177 WP_000132614.1 NZ_CP024650:c4961914-4961573 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T88177 NZ_CP024650:c4961914-4961573 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT88177 NZ_CP024650:4961909-4961985 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin