Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4346602..4347204 Replicon chromosome
Accession NZ_CP024650
Organism Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag CDW44_RS22025 Protein ID WP_000897305.1
Coordinates 4346602..4346913 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag CDW44_RS22030 Protein ID WP_000356397.1
Coordinates 4346914..4347204 (+) Length 97 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDW44_RS22000 4342516..4343115 + 600 WP_001298594.1 glucose-1-phosphatase -
CDW44_RS22005 4343109..4343981 + 873 WP_000920762.1 virulence factor BrkB family protein -
CDW44_RS22010 4343978..4344415 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
CDW44_RS22015 4344460..4345401 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
CDW44_RS22020 4345465..4346373 - 909 WP_001298596.1 alpha/beta hydrolase -
CDW44_RS22025 4346602..4346913 + 312 WP_000897305.1 hypothetical protein Toxin
CDW44_RS22030 4346914..4347204 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
CDW44_RS22040 4347809..4348027 + 219 WP_001298592.1 ribbon-helix-helix domain-containing protein -
CDW44_RS22045 4348242..4349171 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
CDW44_RS22050 4349168..4349803 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
CDW44_RS22055 4349800..4350702 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(36-85)


Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12172.16 Da        Isoelectric Point: 9.7791

>T88174 WP_000897305.1 NZ_CP024650:4346602-4346913 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T88174 NZ_CP024650:4346602-4346913 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT88174 WP_000356397.1 NZ_CP024650:4346914-4347204 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT88174 NZ_CP024650:4346914-4347204 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7


Antitoxin

Source ID Structure

References