Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2148609..2149111 Replicon chromosome
Accession NZ_CP024650
Organism Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag CDW44_RS11090 Protein ID WP_000767822.1
Coordinates 2148609..2148863 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CDW44_RS11095 Protein ID WP_001259255.1
Coordinates 2148860..2149111 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDW44_RS11070 2145102..2146460 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
CDW44_RS11075 2146450..2146512 - 63 WP_010723108.1 membrane protein YoeI -
CDW44_RS11080 2146727..2147656 - 930 WP_000803366.1 LysR family transcriptional regulator -
CDW44_RS11085 2147702..2148526 - 825 WP_000754733.1 SDR family oxidoreductase -
CDW44_RS11090 2148609..2148863 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CDW44_RS11095 2148860..2149111 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CDW44_RS11100 2149394..2149444 + 51 WP_001364200.1 his operon leader peptide -
CDW44_RS11110 2149590..2150489 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CDW44_RS11115 2150495..2151799 + 1305 WP_001297912.1 histidinol dehydrogenase -
CDW44_RS11120 2151796..2152866 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
CDW44_RS11125 2152866..2153933 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T88167 WP_000767822.1 NZ_CP024650:c2148863-2148609 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T88167 NZ_CP024650:c2148863-2148609 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT88167 WP_001259255.1 NZ_CP024650:c2149111-2148860 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT88167 NZ_CP024650:c2149111-2148860 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References