Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 591592..592271 Replicon chromosome
Accession NZ_CP024650
Organism Escherichia coli strain BH100 substr. MG2014

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag CDW44_RS02965 Protein ID WP_000057523.1
Coordinates 591592..591894 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag CDW44_RS02970 Protein ID WP_000806442.1
Coordinates 591930..592271 (+) Length 114 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDW44_RS02935 586968..588620 + 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
CDW44_RS02940 588658..589161 - 504 WP_000667000.1 hypothetical protein -
CDW44_RS02945 589158..589958 - 801 WP_000439798.1 hypothetical protein -
CDW44_RS02950 589982..590461 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
CDW44_RS02960 590665..591459 - 795 WP_023152160.1 TraB/GumN family protein -
CDW44_RS02965 591592..591894 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CDW44_RS02970 591930..592271 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
CDW44_RS02975 592329..594833 - 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
CDW44_RS02980 595095..596027 + 933 WP_000883025.1 glutaminase A -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 582514..601090 18576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-98)

Antitoxin

(1-100)


Sequences


Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T88155 WP_000057523.1 NZ_CP024650:591592-591894 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T88155 NZ_CP024650:591592-591894 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT88155 WP_000806442.1 NZ_CP024650:591930-592271 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT88155 NZ_CP024650:591930-592271 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGTCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PK60


Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References