Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 215278..215935 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP024641 | ||
| Organism | Klebsiella michiganensis strain F107 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | CHQ91_RS01275 | Protein ID | WP_000270043.1 |
| Coordinates | 215278..215628 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | CHQ91_RS01280 | Protein ID | WP_000124640.1 |
| Coordinates | 215633..215935 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CHQ91_RS01225 | 210292..210405 | - | 114 | WP_048336589.1 | small membrane protein | - |
| CHQ91_RS01230 | 210472..211488 | + | 1017 | WP_014839893.1 | IS5-like element IS5 family transposase | - |
| CHQ91_RS01235 | 211752..212249 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| CHQ91_RS01240 | 212252..212740 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| CHQ91_RS01245 | 212837..213172 | + | 336 | WP_000683476.1 | hypothetical protein | - |
| CHQ91_RS01250 | 213187..213657 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| CHQ91_RS01255 | 213650..214021 | - | 372 | WP_000516916.1 | hypothetical protein | - |
| CHQ91_RS01260 | 214032..214226 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| CHQ91_RS01270 | 214567..215115 | - | 549 | WP_001061574.1 | transcriptional regulator | - |
| CHQ91_RS01275 | 215278..215628 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| CHQ91_RS01280 | 215633..215935 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| CHQ91_RS01285 | 215962..216255 | - | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| CHQ91_RS01290 | 216343..216615 | - | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| CHQ91_RS01295 | 216673..217200 | - | 528 | WP_001236377.1 | thermonuclease family protein | - |
| CHQ91_RS01305 | 217431..218288 | - | 858 | WP_001167032.1 | hypothetical protein | - |
| CHQ91_RS01310 | 218275..218505 | - | 231 | WP_000972663.1 | hypothetical protein | - |
| CHQ91_RS01315 | 218505..219023 | - | 519 | WP_000210756.1 | nitrite reductase | - |
| CHQ91_RS01320 | 219020..219466 | - | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| CHQ91_RS01325 | 219466..219825 | - | 360 | WP_000422768.1 | hypothetical protein | - |
| CHQ91_RS01330 | 219882..220310 | - | 429 | WP_000591074.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 | - | 1..308586 | 308586 | |
| - | flank | IS/Tn | - | - | 210508..211488 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T88122 WP_000270043.1 NZ_CP024641:215278-215628 [Klebsiella michiganensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T88122 NZ_CP024641:215278-215628 [Klebsiella michiganensis]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT88122 WP_000124640.1 NZ_CP024641:215633-215935 [Klebsiella michiganensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT88122 NZ_CP024641:215633-215935 [Klebsiella michiganensis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|