Detailed information of TA system TA088097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2364692..2365277
• Replicon: chromosome
• Accession: NZ_CP024620
• Organism: Acinetobacter indicus strain SGAir0564

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3L0E6
• Locus tag: CTZ23_RS11435
• Protein ID: WP_002126330.1
• Coordinates: 2364692..2365012 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CTZ23_RS11440
• Protein ID: WP_002126333.1
• Coordinates: 2365005..2365277 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTZ23_RS11415	2360149..2360715	-	567	WP_016659854.1	alpha/beta hydrolase	-
CTZ23_RS11420	2360887..2362296	-	1410	WP_005175901.1	signal recognition particle protein	-
CTZ23_RS11425	2362435..2363244	+	810	WP_016659852.1	cytochrome c biogenesis protein CcsA	-
CTZ23_RS11430	2363503..2364480	-	978	WP_104989017.1	TerC family protein	-
CTZ23_RS11435	2364692..2365012	+	321	WP_002126330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTZ23_RS11440	2365005..2365277	+	273	WP_002126333.1	helix-turn-helix domain-containing protein	Antitoxin
CTZ23_RS11445	2365361..2366002	-	642	WP_104989018.1	hypothetical protein	-
CTZ23_RS11450	2366082..2366390	+	309	Protein_2224	type VI secretion system tip protein VgrG	-
CTZ23_RS11460	2367206..2367502	+	297	WP_104989400.1	hypothetical protein	-
CTZ23_RS11465	2367517..2368029	+	513	WP_104989401.1	hypothetical protein	-
CTZ23_RS11475	2368596..2368826	+	231	WP_004779823.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CTZ23_RS11480	2368823..2369218	+	396	WP_104989019.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	bfmS / bfmR / pilT / pilU / tufA	2275185..2507411	232226
	-	flank	IS/Tn	-	-	2369252..2370460	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12191.98 Da
• Isoelectric Point: 7.9834

>T88097 WP_002126330.1 NZ_CP024620:2364692-2365012 [Acinetobacter indicus]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVNEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T88097 NZ_CP024620:2364692-2365012 [Acinetobacter indicus]
ATGTTATTCATTGAAACCAGTATCTTTACCAAGCAAATCAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAACA
AGATCTATTGGTTCAGCCAGATAAAGGTGACTTAATCAAGAATGGCGGTGGCATTCGAAAAGTACGTTGTGCTCAAGGGA
ATAAAGGCAAAAGTGGCGGTATCAGAGTAATTTACTATTGGGTAAATGAGGATGATCAAATCTTTTTCTTGGTGGCTTAT
CCAAAATCTGTAAAAGATAATCTAACGGATAAAGAAACCGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10177.62 Da
• Isoelectric Point: 9.8622

>AT88097 WP_002126333.1 NZ_CP024620:2365005-2365277 [Acinetobacter indicus]
MDNNLFDDLVASIKEAGAIKRKEIQASRTTELKLPDIKEVREKTGLSQSEFAARLHISHRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT88097 NZ_CP024620:2365005-2365277 [Acinetobacter indicus]
ATGGATAACAACTTATTTGATGACTTAGTTGCTTCGATTAAAGAAGCAGGAGCAATCAAGCGCAAGGAGATCCAAGCTAG
TCGAACGACCGAACTTAAATTACCAGATATTAAGGAAGTGCGAGAAAAAACTGGTTTAAGTCAAAGCGAATTTGCAGCAC
GTCTCCACATTAGCCATCGTACTCTGCAAAACTGGGAACAAGGCCGACGCTATCCAACAGGACCAGCAGCAACATTGATT
CGAATTTTAGATGCACATCCTAGTCTTATTTAA

Structures

Toxin

• AlphaFold DB: A0A3F3L0E6