Detailed information of TA system TA088092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4284671..4285470
• Replicon: chromosome
• Accession: NZ_CP024618
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3I9P420
• Locus tag: CR916_RS24645
• Protein ID: WP_000347271.1
• Coordinates: 4285006..4285470 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: CR916_RS24640
• Protein ID: WP_062882489.1
• Coordinates: 4284671..4285006 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS24625	4280456..4281226	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CR916_RS24630	4281242..4282576	-	1335	WP_062882487.1	galactarate/glucarate/glycerate transporter GarP	-
CR916_RS24635	4282951..4284522	+	1572	WP_062882488.1	galactarate dehydratase	-
CR916_RS24640	4284671..4285006	+	336	WP_062882489.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CR916_RS24645	4285006..4285470	+	465	WP_000347271.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CR916_RS24650	4285525..4286334	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CR916_RS24660	4286583..4287863	+	1281	Protein_4372	tagatose-bisphosphate aldolase subunit KbaZ	-
CR916_RS24665	4287886..4288359	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CR916_RS24670	4288370..4289149	+	780	WP_062882490.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CR916_RS24675	4289139..4290017	+	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4274035..4285470	11435

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17849.25 Da
• Isoelectric Point: 9.6924

>T88092 WP_000347271.1 NZ_CP024618:4285006-4285470 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH

Nucleotide

• Length: 465 bp

>T88092 NZ_CP024618:4285006-4285470 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12375.97 Da
• Isoelectric Point: 4.8616

>AT88092 WP_062882489.1 NZ_CP024618:4284671-4285006 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT88092 NZ_CP024618:4284671-4285006 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3I9P420