Detailed information of TA system TA088073

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralR-sib/-
• Location: 2073469..2073840
• Replicon: chromosome
• Accession: NZ_CP024618
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: CR916_RS12360
• Protein ID: WP_042853000.1
• Coordinates: 2073646..2073840 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	2073469..2073647 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS12330	2069221..2069394	+	174	WP_001296046.1	hypothetical protein	-
CR916_RS12335	2069424..2070797	+	1374	WP_024226008.1	ATP-dependent RNA helicase DbpA	-
CR916_RS12340	2070926..2071861	-	936	WP_096851542.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CR916_RS12345	2071913..2073148	-	1236	WP_096851543.1	site-specific integrase	-
CR916_RS12350	2073150..2073365	-	216	WP_000079604.1	excisionase XisR	-
CR916_RS12355	2073465..2073653	-	189	WP_001356607.1	DUF1187 family protein	-
-	2073469..2073647	+	179	NuclAT_0	-	Antitoxin
-	2073469..2073647	+	179	NuclAT_0	-	Antitoxin
-	2073469..2073647	+	179	NuclAT_0	-	Antitoxin
-	2073469..2073647	+	179	NuclAT_0	-	Antitoxin
CR916_RS12360	2073646..2073840	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CR916_RS12365	2073897..2074706	-	810	WP_000166315.1	recombination protein RecT	-
CR916_RS12370	2074699..2077368	-	2670	WP_000102194.1	exodeoxyribonuclease VIII	-
CR916_RS12375	2077449..2077625	-	177	WP_000245534.1	hypothetical protein	-
CR916_RS12380	2077619..2077840	-	222	WP_000560226.1	killing protein KilR	-
CR916_RS12385	2077887..2078735	-	849	WP_000887681.1	Rha family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2071913..2114692	42779

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T88073 WP_042853000.1 NZ_CP024618:c2073840-2073646 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T88073 NZ_CP024618:c2073840-2073646 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT88073 NZ_CP024618:2073469-2073647 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin