Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 762461..763002 Replicon chromosome
Accession NZ_CP024618
Organism Escherichia coli strain SMN152SH1

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CR916_RS05015 Protein ID WP_000615979.1
Coordinates 762461..762739 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CR916_RS05020 Protein ID WP_000729704.1
Coordinates 762742..763002 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR916_RS05000 760043..760621 + 579 WP_062882565.1 D-sedoheptulose 7-phosphate isomerase -
CR916_RS05005 760827..761594 + 768 WP_001365365.1 class II glutamine amidotransferase -
CR916_RS05010 761565..762305 - 741 WP_001225679.1 murein L,D-transpeptidase -
CR916_RS05015 762461..762739 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CR916_RS05020 762742..763002 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CR916_RS30625 763188..763298 + 111 Protein_753 peptidoglycan endopeptidase -
CR916_RS05025 763289..763650 - 362 Protein_754 FHIPEP family type III secretion protein -
CR916_RS05030 763622..764407 + 786 WP_062882563.1 putative lateral flagellar export/assembly protein LafU -
CR916_RS05035 764478..765533 + 1056 WP_001226164.1 DNA polymerase IV -
CR916_RS05040 765530..765769 + 240 Protein_757 GNAT family N-acetyltransferase -
CR916_RS05050 766538..766756 + 219 Protein_759 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T88068 WP_000615979.1 NZ_CP024618:c762739-762461 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T88068 NZ_CP024618:c762739-762461 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT88068 WP_000729704.1 NZ_CP024618:c763002-762742 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT88068 NZ_CP024618:c763002-762742 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References