Detailed information of TA system TA088066

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 380638..381050
• Replicon: chromosome
• Accession: NZ_CP024618
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0B1K116
• Locus tag: CR916_RS03050
• Protein ID: WP_000132623.1
• Coordinates: 380638..380979 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	380974..381050 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS03035	376647..378059	-	1413	WP_000199311.1	PLP-dependent aminotransferase family protein	-
CR916_RS03040	378236..378400	+	165	WP_000394278.1	DUF1127 domain-containing protein	-
CR916_RS03045	378498..380591	+	2094	WP_062881594.1	DUF262 domain-containing protein	-
CR916_RS03050	380638..380979	-	342	WP_000132623.1	endoribonuclease SymE	Toxin
-	380974..381050	+	77	NuclAT_29	-	Antitoxin
-	380974..381050	+	77	NuclAT_29	-	Antitoxin
-	380974..381050	+	77	NuclAT_29	-	Antitoxin
-	380974..381050	+	77	NuclAT_29	-	Antitoxin
-	380974..381050	+	77	NuclAT_30	-	Antitoxin
-	380974..381050	+	77	NuclAT_30	-	Antitoxin
-	380974..381050	+	77	NuclAT_30	-	Antitoxin
-	380974..381050	+	77	NuclAT_30	-	Antitoxin
CR916_RS03060	381968..385231	-	3264	WP_062881360.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12323.21 Da
• Isoelectric Point: 9.5818

>T88066 WP_000132623.1 NZ_CP024618:c380979-380638 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMKGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVREFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T88066 NZ_CP024618:c380979-380638 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTATCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCTA
CCGGCACGGCGGTAGATGTCAAAGTGATGAAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAG
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCGGGAATTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT88066 NZ_CP024618:380974-381050 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0B1K116

Antitoxin