Detailed information of TA system TA088059

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41371..41625
• Replicon: plasmid pO177A1
• Accession: NZ_CP024617
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CR916_RS00615
• Protein ID: WP_001351576.1
• Coordinates: 41419..41625 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	41371..41432 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS00595	39086..39832	+	747	WP_062881715.1	conjugal transfer pilus acetylase TraX	-
CR916_RS00600	39887..40447	+	561	WP_062881716.1	fertility inhibition protein FinO	-
CR916_RS00605	40583..40795	+	213	WP_001299730.1	ANR family transcriptional regulator	-
CR916_RS30560	41041..41115	+	75	Protein_43	endonuclease	-
-	41371..41432	-	62	NuclAT_0	-	Antitoxin
-	41371..41432	-	62	NuclAT_0	-	Antitoxin
-	41371..41432	-	62	NuclAT_0	-	Antitoxin
-	41371..41432	-	62	NuclAT_0	-	Antitoxin
CR916_RS00615	41419..41625	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
CR916_RS00620	41909..42166	+	258	WP_000083845.1	replication regulatory protein RepA	-
CR916_RS00630	42398..42472	+	75	WP_001442103.1	RepA leader peptide Tap	-
CR916_RS00635	42465..42947	+	483	WP_001523473.1	hypothetical protein	-
CR916_RS00640	42940..43797	+	858	WP_096851680.1	incFII family plasmid replication initiator RepA	-
CR916_RS00650	44709..44993	+	285	WP_062881718.1	ribbon-helix-helix domain-containing protein	-
CR916_RS00655	44993..45268	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / hlyD / hlyB / hlyA / hlyC	1..88896	88896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T88059 WP_001351576.1 NZ_CP024617:41419-41625 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T88059 NZ_CP024617:41419-41625 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT88059 NZ_CP024617:c41432-41371 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin