Detailed information of TA system TA087927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3894599..3895196
• Replicon: chromosome
• Accession: NZ_CP024545
• Organism: Klebsiella pneumoniae strain INF059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: CTI65_RS19675
• Protein ID: WP_004893639.1
• Coordinates: 3894879..3895196 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: CTI65_RS19670
• Protein ID: WP_004142561.1
• Coordinates: 3894599..3894886 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI65_RS19640	3890806..3891054	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
CTI65_RS19645	3891072..3891413	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
CTI65_RS19650	3891444..3892559	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
CTI65_RS19655	3892739..3893320	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
CTI65_RS19660	3893320..3893688	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
CTI65_RS19665	3893808..3894461	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
CTI65_RS19670	3894599..3894886	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
CTI65_RS19675	3894879..3895196	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTI65_RS19680	3895381..3896424	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
CTI65_RS19690	3897094..3897960	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
CTI65_RS19695	3898069..3899496	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T87927 WP_004893639.1 NZ_CP024545:c3895196-3894879 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T87927 NZ_CP024545:c3895196-3894879 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT87927 WP_004142561.1 NZ_CP024545:c3894886-3894599 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT87927 NZ_CP024545:c3894886-3894599 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1