Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 49436..49700 Replicon plasmid unnamed2
Accession NZ_CP024481
Organism Escherichia coli strain 2011C-4315

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag A5958_RS29240 Protein ID WP_001303307.1
Coordinates 49548..49700 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 49436..49498 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5958_RS29225 44675..46966 - 2292 WP_072279833.1 conjugal transfer protein TrbC -
A5958_RS29230 46959..48029 - 1071 WP_052895276.1 IncI1-type conjugal transfer protein TrbB -
A5958_RS29235 48048..49256 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 49436..49498 - 63 NuclAT_0 - Antitoxin
- 49436..49498 - 63 NuclAT_0 - Antitoxin
- 49436..49498 - 63 NuclAT_0 - Antitoxin
- 49436..49498 - 63 NuclAT_0 - Antitoxin
A5958_RS29240 49548..49700 + 153 WP_001303307.1 Hok/Gef family protein Toxin
A5958_RS29245 49772..50023 - 252 WP_001291965.1 hypothetical protein -
A5958_RS29250 50164..50814 + 651 WP_024244070.1 IS66 family insertion sequence hypothetical protein -
A5958_RS29255 50814..51161 + 348 WP_024244069.1 IS66 family insertion sequence element accessory protein TnpB -
A5958_RS29260 51181..52751 + 1571 Protein_64 IS66 family transposase -
A5958_RS29265 52895..53034 + 140 Protein_65 transposase domain-containing protein -
A5958_RS29270 53076..53603 - 528 WP_000182867.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..105489 105489


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T87783 WP_001303307.1 NZ_CP024481:49548-49700 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T87783 NZ_CP024481:49548-49700 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT87783 NZ_CP024481:c49498-49436 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References