Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5239053..5239555 Replicon chromosome
Accession NZ_CP024479
Organism Escherichia coli strain 2011C-4315

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A5958_RS27825 Protein ID WP_000767829.1
Coordinates 5239301..5239555 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A5958_RS27820 Protein ID WP_001259255.1
Coordinates 5239053..5239304 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5958_RS27790 5234231..5235298 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
A5958_RS27795 5235298..5236368 - 1071 WP_000108980.1 histidinol-phosphate transaminase -
A5958_RS27800 5236365..5237669 - 1305 WP_001298841.1 histidinol dehydrogenase -
A5958_RS27805 5237675..5238574 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
A5958_RS27815 5238720..5238770 - 51 WP_001368802.1 his operon leader peptide -
A5958_RS27820 5239053..5239304 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A5958_RS27825 5239301..5239555 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A5958_RS27830 5239638..5240462 + 825 WP_000754737.1 SDR family oxidoreductase -
A5958_RS27835 5240508..5241437 + 930 WP_000803351.1 LysR family transcriptional regulator -
A5958_RS27840 5241652..5241714 + 63 WP_010723108.1 membrane protein YoeI -
A5958_RS27845 5241704..5243062 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A5958_RS27850 5243241..5244299 + 1059 WP_000492346.1 transport protein YeeE -
A5958_RS27855 5244313..5244540 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T87780 WP_000767829.1 NZ_CP024479:5239301-5239555 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87780 NZ_CP024479:5239301-5239555 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87780 WP_001259255.1 NZ_CP024479:5239053-5239304 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87780 NZ_CP024479:5239053-5239304 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References