Detailed information of TA system TA087772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3035278..3035880
• Replicon: chromosome
• Accession: NZ_CP024479
• Organism: Escherichia coli strain 2011C-4315

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A5958_RS16415
• Protein ID: WP_000897305.1
• Coordinates: 3035569..3035880 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A5958_RS16410
• Protein ID: WP_000356397.1
• Coordinates: 3035278..3035568 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5958_RS16375	3030844..3031479	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A5958_RS16380	3031476..3032405	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A5958_RS16390	3032804..3033784	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
A5958_RS16395	3034013..3034255	-	243	WP_001086388.1	hypothetical protein	-
A5958_RS16400	3034474..3034692	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
A5958_RS16410	3035278..3035568	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A5958_RS16415	3035569..3035880	-	312	WP_000897305.1	hypothetical protein	Toxin
A5958_RS16420	3036109..3037017	+	909	WP_001162704.1	alpha/beta hydrolase	-
A5958_RS16425	3037081..3038022	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
A5958_RS16430	3038067..3038504	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A5958_RS16435	3038501..3039373	-	873	WP_000920762.1	virulence factor BrkB family protein	-
A5958_RS16440	3039367..3039966	-	600	WP_001295269.1	glucose-1-phosphatase	-
A5958_RS16445	3040065..3040850	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3032804..3033784	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T87772 WP_000897305.1 NZ_CP024479:c3035880-3035569 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T87772 NZ_CP024479:c3035880-3035569 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT87772 WP_000356397.1 NZ_CP024479:c3035568-3035278 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT87772 NZ_CP024479:c3035568-3035278 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7