Detailed information of TA system TA087736

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78632..78885
• Replicon: plasmid unnamed2
• Accession: NZ_CP024475
• Organism: Shigella flexneri 7b strain 94-3007

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CPZ98_RS24205
• Protein ID: WP_001312851.1
• Coordinates: 78736..78885 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	78632..78691 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ98_RS24165 (74566)	74566..75312	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
CPZ98_RS24170 (75367)	75367..75927	+	561	WP_033807966.1	fertility inhibition protein FinO	-
CPZ98_RS24175 (76059)	76059..76271	+	213	WP_001301409.1	ANR family transcriptional regulator	-
CPZ98_RS24180 (76517)	76517..76978	+	462	WP_001233850.1	thermonuclease family protein	-
CPZ98_RS24185 (77024)	77024..77233	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
CPZ98_RS24190 (77271)	77271..77861	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
CPZ98_RS24195 (78016)	78016..78489	+	474	WP_016240489.1	hypothetical protein	-
- (78632)	78632..78691	-	60	NuclAT_1	-	Antitoxin
- (78632)	78632..78691	-	60	NuclAT_1	-	Antitoxin
- (78632)	78632..78691	-	60	NuclAT_1	-	Antitoxin
- (78632)	78632..78691	-	60	NuclAT_1	-	Antitoxin
CPZ98_RS24205 (78736)	78736..78885	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
CPZ98_RS24210 (79170)	79170..79418	+	249	WP_000083839.1	replication regulatory protein RepA	-
CPZ98_RS24220 (79663)	79663..79737	+	75	WP_001365705.1	RepA leader peptide Tap	-
CPZ98_RS24225 (79730)	79730..80587	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
CPZ98_RS24235 (81526)	81526..82179	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CPZ98_RS24240 (82272)	82272..82529	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B)	-	1..82833	82833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T87736 WP_001312851.1 NZ_CP024475:78736-78885 [Shigella flexneri 7b]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T87736 NZ_CP024475:78736-78885 [Shigella flexneri 7b]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT87736 NZ_CP024475:c78691-78632 [Shigella flexneri 7b]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin