Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 4027378..4027603 Replicon chromosome
Accession NZ_CP024473
Organism Shigella flexneri 7b strain 94-3007

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag CPZ98_RS20705 Protein ID WP_000813254.1
Coordinates 4027378..4027533 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 4027545..4027603 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ98_RS20640 4022690..4023040 - 351 WP_005049241.1 IS66 family insertion sequence element accessory protein TnpB -
CPZ98_RS20645 4023037..4023711 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -
CPZ98_RS20650 4023810..4024025 - 216 WP_000839572.1 class II holin family protein -
CPZ98_RS20680 4024821..4025509 - 689 Protein_3972 bacteriophage antitermination protein Q -
CPZ98_RS20685 4025506..4025871 - 366 WP_128832266.1 RusA family crossover junction endodeoxyribonuclease -
CPZ98_RS20690 4025872..4026930 - 1059 WP_001265248.1 DUF968 domain-containing protein -
CPZ98_RS20695 4026932..4027210 - 279 WP_011069426.1 hypothetical protein -
CPZ98_RS20705 4027378..4027533 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 4027545..4027603 + 59 - - Antitoxin
CPZ98_RS20720 4028185..4028601 - 417 WP_005069274.1 hypothetical protein -
CPZ98_RS20725 4028628..4028768 + 141 Protein_3978 DUF4224 domain-containing protein -
CPZ98_RS20730 4028768..4029808 + 1041 WP_005096324.1 tyrosine-type recombinase/integrase -
CPZ98_RS20740 4030019..4030816 + 798 WP_024260703.1 DgsA anti-repressor MtfA -
CPZ98_RS20750 4031154..4032416 + 1263 Protein_3981 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 3994078..4035724 41646


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T87731 WP_000813254.1 NZ_CP024473:c4027533-4027378 [Shigella flexneri 7b]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T87731 NZ_CP024473:c4027533-4027378 [Shigella flexneri 7b]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT87731 NZ_CP024473:4027545-4027603 [Shigella flexneri 7b]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References