Detailed information of TA system TA087720

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3371038..3371263
• Replicon: chromosome
• Accession: NZ_CP024473
• Organism: Shigella flexneri 7b strain 94-3007

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CPZ98_RS17035
• Protein ID: WP_000813254.1
• Coordinates: 3371108..3371263 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3371038..3371096 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ98_RS26500	3367039..3367208	+	170	Protein_3270	hypothetical protein	-
CPZ98_RS17000	3367354..3368097	+	744	WP_000788999.1	ATP-binding protein	-
CPZ98_RS17005	3368112..3368534	+	423	WP_001118167.1	DUF977 family protein	-
CPZ98_RS17010	3368592..3368948	+	357	WP_005048249.1	hypothetical protein	-
CPZ98_RS17015	3369041..3369259	+	219	WP_000256998.1	DUF4014 family protein	-
CPZ98_RS17020	3369261..3369626	+	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
CPZ98_RS17025	3369623..3370288	+	666	WP_000208062.1	hypothetical protein	-
CPZ98_RS17030	3370288..3370653	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	3371038..3371096	-	59	-	-	Antitoxin
CPZ98_RS17035	3371108..3371263	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
CPZ98_RS17050	3372600..3373199	+	600	WP_000940329.1	DUF1367 family protein	-
CPZ98_RS17055	3373199..3373489	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
CPZ98_RS17060	3373486..3374040	+	555	WP_000640143.1	DUF1133 family protein	-
CPZ98_RS17065	3374193..3374366	+	174	WP_000504450.1	hypothetical protein	-
CPZ98_RS17070	3374428..3375584	+	1157	WP_094081550.1	IS3-like element IS600 family transposase	-
CPZ98_RS17075	3375577..3376137	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	3360475..3407755	47280

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T87720 WP_000813254.1 NZ_CP024473:3371108-3371263 [Shigella flexneri 7b]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T87720 NZ_CP024473:3371108-3371263 [Shigella flexneri 7b]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT87720 NZ_CP024473:c3371096-3371038 [Shigella flexneri 7b]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin