Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2667676..2668204 Replicon chromosome
Accession NZ_CP024473
Organism Shigella flexneri 7b strain 94-3007

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag CPZ98_RS13235 Protein ID WP_005061113.1
Coordinates 2667974..2668204 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag CPZ98_RS13230 Protein ID WP_000147081.1
Coordinates 2667676..2667984 (-) Length 103 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ98_RS13180 (2662787) 2662787..2662942 + 156 WP_014532198.1 type I toxin-antitoxin system toxin HokD -
CPZ98_RS13185 (2663192) 2663192..2663479 + 288 WP_000687442.1 hypothetical protein -
CPZ98_RS13190 (2663518) 2663518..2663886 + 369 Protein_2536 DUF1367 family protein -
CPZ98_RS13200 (2665151) 2665151..2665384 + 234 Protein_2538 DUF1367 family protein -
CPZ98_RS13205 (2665384) 2665384..2665674 + 291 WP_000228005.1 DUF1364 domain-containing protein -
CPZ98_RS13210 (2665671) 2665671..2665781 + 111 Protein_2540 DUF1133 family protein -
CPZ98_RS13215 (2665858) 2665858..2667026 + 1169 WP_000747032.1 IS3-like element IS911 family transposase -
CPZ98_RS13220 (2667051) 2667051..2667461 + 411 WP_000814621.1 recombination protein NinB -
CPZ98_RS13225 (2667458) 2667458..2667634 + 177 WP_001252556.1 NinE family protein -
CPZ98_RS13230 (2667676) 2667676..2667984 - 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
CPZ98_RS13235 (2667974) 2667974..2668204 - 231 WP_005061113.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CPZ98_RS13240 (2668217) 2668217..2668675 + 459 WP_000961311.1 ash family protein -
CPZ98_RS13245 (2668733) 2668733..2669185 + 453 WP_232046050.1 Rha family transcriptional regulator -
CPZ98_RS13250 (2669182) 2669182..2669415 + 234 WP_000618004.1 hypothetical protein -
CPZ98_RS13255 (2669402) 2669402..2670310 + 909 WP_001247840.1 hypothetical protein -
CPZ98_RS13260 (2670321) 2670321..2671199 + 879 WP_000988183.1 ATP-binding protein -
CPZ98_RS13265 (2671196) 2671196..2672587 + 1392 WP_000211324.1 replicative DNA helicase -
CPZ98_RS13270 (2672584) 2672584..2672841 + 258 WP_001064799.1 hypothetical protein -
CPZ98_RS13275 (2672936) 2672936..2673136 + 201 WP_000111769.1 protein ninH -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH4.5 2615155..2705969 90814


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-70)

Antitoxin

(44-97)


Sequences


Toxin        


Download         Length: 77 a.a.        Molecular weight: 8665.12 Da        Isoelectric Point: 10.5308

>T87716 WP_005061113.1 NZ_CP024473:c2668204-2667974 [Shigella flexneri 7b]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW

Download         Length: 231 bp

>T87716 NZ_CP024473:c2668204-2667974 [Shigella flexneri 7b]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT87716 WP_000147081.1 NZ_CP024473:c2667984-2667676 [Shigella flexneri 7b]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT87716 NZ_CP024473:c2667984-2667676 [Shigella flexneri 7b]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References