Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2370958..2371576 Replicon chromosome
Accession NZ_CP024473
Organism Shigella flexneri 7b strain 94-3007

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CPZ98_RS11665 Protein ID WP_001291435.1
Coordinates 2370958..2371176 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag CPZ98_RS11670 Protein ID WP_000344797.1
Coordinates 2371202..2371576 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ98_RS11635 (2366850) 2366850..2367161 - 312 WP_000409911.1 MGMT family protein -
CPZ98_RS11645 (2367540) 2367540..2367893 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CPZ98_RS11650 (2367935) 2367935..2369485 - 1551 WP_005106783.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CPZ98_RS11655 (2369649) 2369649..2370119 - 471 WP_000136192.1 YlaC family protein -
CPZ98_RS11660 (2370235) 2370235..2370786 - 552 WP_000102577.1 maltose O-acetyltransferase -
CPZ98_RS11665 (2370958) 2370958..2371176 - 219 WP_001291435.1 HHA domain-containing protein Toxin
CPZ98_RS11670 (2371202) 2371202..2371576 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
CPZ98_RS11675 (2372122) 2372122..2375271 - 3150 WP_001132485.1 efflux RND transporter permease AcrB -
CPZ98_RS11680 (2375294) 2375294..2376487 - 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2365492..2366820 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T87715 WP_001291435.1 NZ_CP024473:c2371176-2370958 [Shigella flexneri 7b]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T87715 NZ_CP024473:c2371176-2370958 [Shigella flexneri 7b]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT87715 WP_000344797.1 NZ_CP024473:c2371576-2371202 [Shigella flexneri 7b]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT87715 NZ_CP024473:c2371576-2371202 [Shigella flexneri 7b]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References